Human glutamine synthetase gene and drug-metabolizing CYP3A4 gene introduced Hep G2 cells.
Biosafety Level:
1
Morphology:
fibroblast-like
Medium:
RDF medium containing glucose (2.58 g/L), glutamine (333 mg/L), and 10% dialyzed fetal bovine serum. RDF medium is a 2:1:1 mixture of RPMI1640, DMEM, Ham's F12 without glucose and glutamine.
